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aleponce4/README.md

Alex Ponce-Flores

I work across bioinformatics, scientific software, and applied machine learning. I build Python and R workflows for genomics, image analysis, and automated instrument acquisition, with an emphasis on reproducibility, quality control, and quantitative validation.

Portfolio · LinkedIn · ORCID

Researcher I working in bioinformatics at the University of Tennessee Health Science Center, and Co-Founder / Scientific Data, Software & Applied ML Lead at Onteko.

Areas of work

  • Scientific software and automation — instrument integration, automated acquisition, data processing, and interactive research tools.
  • Bioinformatics and image analysis — RNA-seq, viral genomics, microscopy segmentation, and quantitative evaluation.
  • Reproducible data and applied ML — Python/R pipelines, SLURM/HPC, statistical modeling, automated tests, and traceable results.

Selected work

ProLIBSpector — public edition — Python software for automated LIBS spectroscopy acquisition, spectral processing, and spatial mapping, with simulated devices for reproducible examples. The public edition demonstrates selected parts of the system; ongoing development is maintained in a private implementation.

Image segmentation and validation — Xenium microscopy — Evaluated pretrained Cellpose-SAM with tiled GPU inference and quantitative checks of RNA assignment. Retained the instrument segmentation when expanded masks increased off-type signal. Images, evaluation, and recorded GPU timings are shown in the portfolio.

Lab results browser with local AI assistance — R/Shiny software connecting RNA-seq results, gene networks, and animal-study outcomes, with an optional local assistant. The portfolio includes selected de-identified screenshots, architecture, and a 20-question case evaluation. Code is private because it works with unpublished lab data; happy to walk through the architecture and implementation.

Viral intrahost variant workflow — Containerized Nextflow DSL2 workflow for viral variant calling, consensus generation, selection analysis, and haplotype reconstruction, with automated tests and documented local/HPC execution.

More work

Background and contact

M.S. Bioinformatics, Brandeis University (2024) · B.S. Biology, University of Memphis (2021).

Open to roles in scientific software, bioinformatics, scientific data analysis, automation, and applied ML.

aleponce92@gmail.com

See individual repositories for licensing.

Pinned Loading

  1. viral-intrahost-variant-workflow viral-intrahost-variant-workflow Public

    Containerized Nextflow DSL2 workflow for viral intra-host variant calling (iSNV), quasispecies haplotype reconstruction, and evolutionary selection analysis.

    Python 1

  2. rnaseq-nfcore-wrapper-alphavirus rnaseq-nfcore-wrapper-alphavirus Public

    SLURM execution wrapper and configuration layer for nf-core/rnaseq used in viral RNA-seq projects.

    Python 1

  3. libs-spectroscopy-workbench libs-spectroscopy-workbench Public

    Python software for LIBS spectral processing, baseline correction, elemental line identification, and simulated acquisition.

    Python 13 3

  4. preclinical-study-analysis-shiny preclinical-study-analysis-shiny Public archive

    R/Shiny app for longitudinal mouse study visualization and export from spreadsheet inputs.

    R

  5. lab-bioinfo-templates lab-bioinfo-templates Public

    Reusable virology and genomics analysis templates developed for the Jonsson Lab, with a Quarto gallery and synthetic example data.

    R

  6. akodon-genome-assembly-workflow akodon-genome-assembly-workflow Public

    Genome assembly and annotation pipeline for Akodon using 10x Genomics Supernova and BRAKER-based gene prediction on HPC.

    Shell